By Yuehuei H. An, Kylie L. Martin
Histologists and histochemists needs to spend huge attempt this day to discover the tools necessary to practice bone and cartilage-related study. In instruction manual of Histology tools for Bone and Cartilage, Y.H. An and K.L. Martin have assembled an in depth choice of the most important tools for learning the histology and histomorphometry of bone and cartilage. defined by way of well-experienced researchers, physicians, and technicians, the tools comprise ideas for bone labeling, bone sectioning, histological research, histomorphometric research, radiological exam, and speak to radiography. one of the functions awarded are in situ hybridization of bone and cartilage, radiological exam of calcified tissue with emphasis on bone, and working with histology artifacts in hard-tissue study. There also are histological recommendations for the research of bone-implant interfaces, cartilage-defect fix, and the soft-tissue biomaterial interface. simpler than theoretical, the textual content is easy and easy, with many tables, line drawings, and micro- and macro pictures to demonstrate the content material. complete bibliographies on the finish of every bankruptcy consultant the reader to extra precise information.
complete and updated, the instruction manual of Histology equipment for Bone and Cartilage bargains researchers a pragmatic consultant to all of the significant histological and histomorphometrical tools of bone and cartilage analysis-a gold mine of special suggestions for the coaching of tissue from assortment via fixation, embedding, sectioning, and staining.
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Extra info for Handbook of Histology Methods for Bone and Cartilage
Sample text
Calcif Tissue Int 55:46–52, 1994. 30. Gonzalez del Pino J, Knapp K, Gomez Castresana F, et al: Revascularization of femoral head ischemic necrosis with vascularized bone graft: a CT scan experimental study. Skeletal Radiol 19:197–202, 1990. 31. Gruber HE: Adaptations of Goldner’s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech Histochem 67:30–34, 1992. 32. Gruber HE, Marshall GJ, Nolasco LM, et al: Alkaline and acid phosphatase demonstration in human bone and cartilage: effects of fixation interval and methacrylate embedments.
The cell membranes of damaged, dead, or dying cells are penetrated by propidium iodide or ethidium homodimer, and their nuclei will fluoresce red. 71 Experimental Bone and Cartilage Histology 19 IV. GENERAL CONSIDERATIONS OF HISTOLOGICAL EVALUATION OF BONE AND CARTILAGE A. Histological Evaluation Histological evaluation and characterization of bone and cartilage tissues are normally done with light microscopy. Descriptive histology and histomorphometry are the two main types of histological study.
This is followed by the vascular porosity (on the order of 20 μm), which encompasses the osteonal canals as well as the smaller Volkmann canals. 28 Bone porosity at the next level is associated with the spaces between the collagen and mineral crystals (on the order of 10 nm). 112 42 Walsh et al. Figure 8. Hematoxylin and eosin (H&E) staining (20× objective) at 2 wk in a rat femoral fresh fracture. New woven bone (arrows) as well as the presence of chondroblastic cells “CB” can be seen in the section.