By John J. Bogdanske, Scott Hubbard-Van Stelle, Margaret Rankin Riley, Beth M. Schiffman
DVD textual content and Voice-over Mouse Handling/TransferMouse RestraintOral GavageOne-Handed Injection TechniqueIntraperitoneal Injection (IP) - One personIntraperitoneal Injection (IP) - One-Person Towel MethodSubcutaneous Injection (SQ)Subcutaneous Injection (SQ) - One-Person Towel MethodMaxillary BleedPedal Vein Blood DrawSaphenous Vein Blood DrawTail Vein InjectionRetro-Orbital Bleed - Anesthesia RequiredRetro-Orbital Injections - Anesthesia RequiredEar NotchingEar TaggingMouse Procedural approach Hand-outsIntraperitoneal Injection (IP)Subcutaneous Injection (SQ)Maxillary BleedOral GavagePedal Vei. Read more...
summary: DVD textual content and Voice-over Mouse Handling/TransferMouse RestraintOral GavageOne-Handed Injection TechniqueIntraperitoneal Injection (IP) - One personIntraperitoneal Injection (IP) - One-Person Towel MethodSubcutaneous Injection (SQ)Subcutaneous Injection (SQ) - One-Person Towel MethodMaxillary BleedPedal Vein Blood DrawSaphenous Vein Blood DrawTail Vein InjectionRetro-Orbital Bleed - Anesthesia RequiredRetro-Orbital Injections - Anesthesia RequiredEar NotchingEar TaggingMouse Procedural method Hand-outsIntraperitoneal Injection (IP)Subcutaneous Injection (SQ)Maxillary BleedOral GavagePedal Vei
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Extra resources for Laboratory Mouse Procedural Techniques : Manual and DVD
Recommended skills: You must know and understand the proper methods to restrain a mouse for this technique. Multiple blood draws are possible from the same site as long as the maximum amount of total blood drawn is not exceeded. 5-mm lancet, blood collection tube, 2 × 2 gauze, exam gloves, sharps container. 45 46 laboratory mouse procedural techniques: manual and DVD Albino mice have a convenient landmark shown in (A) that is in the area of the maxillary vein. The circle shows where to insert the lancet.
In a matter of seconds, the blue dye that was injected in this demonstration can be seen in the feet, ears, and tail. 15 ear notching Ear notching or punching is a long-term identification method that can be performed quickly with little pain or distress. Mice are restrained by the scruff, and using an ear punch, holes or notches are placed in the ears following a simple identification chart. Removed tissue can be used for genotyping, possibly replacing the need for a tail biopsy. Care must be taken when placing the punches.
Blood will flow by capillary action into the tube. At the conclusion of the blood withdrawal, release the tension on the facial skin. Close the lid over the eye and gently apply a gauze pad until the bleeding has stopped. Reconfirm normal color and respiration and return the animal to its cage for recovery. Use alternate eyes for successive bleeds. 27 28 laboratory mouse procedural techniques: manual and DVD recommended supplies • Heparinized capillary tube • Tube rack • Gauze • Blood collection tube • Anesthesia Voice-Over 1: This illustration depicts the orbital sinus of the mouse.