By Ado Jorio
This publication covers the improvement of biotechnology in keeping with carbon nanostructures, with a spotlight on nanotubes, addressing additionally fullerenes and amorphous carbons. The ebook is split into 7 chapters, addressing tissue engineering, genetic engineering and remedy, in addition to the environmental and wellbeing and fitness affects of carbon nanostructures.
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De Carvalho Á A. A. C. A. C. br A. A. C. A. br M. br © Springer International Publishing Switzerland 2016 A. 1007/978-3-319-25907-9_3 31 32 H. de Mello Brandão et al. interaction of DNA/RNA with CNT favors their protection from degradation by cytoplasmic nucleases, increasing the integration of the transgene into cell nucleus. Thus, the use of CNTs can be far simpler and less laborious when compared to other techniques to produce GMAs. 1 Introduction Genetic engineering aims to intentionally remove, modify or add genes to DNA molecule in order to improve the development of new therapies and new disease models; to study the mammalian gene function; and to generate genetically modified organisms (GMO) and genetically modified animals (GMA) [1, 2].
These hybrid molecules open up a new perspective for the modification of the genome of the domestic species. 1 Carbon Nanotubes (CNT) as a Gene Transfer Agent Interaction of CNT and DNA Carbon nanotube (CNT) can be easily bound to DNA by non-covalent attachment [86]. The DNA strand spontaneously wraps around the CNT [87, 88]. Gao and Kong [89], using molecular modeling, found that DNA molecules can be 3 Carbon Nanotubes as a DNA Delivery … 41 encapsulated within or around the CNT. The nucleotide bases interact with the CNT by hydrophobic interactions.
Int J Nanomedicine 7:5361–5374 13. Kam NW, Liu Z, Dai H (2005) Functionalization of carbon nanotubes via cleavable disulfide bonds for efficient intracellular delivery of siRNA and potent gene silencing. J Am Chem Soc 127:12492–12493 14. Liu Z, Winters M, Holodniy M, Dai H (2007) siRNA delivery into human T cells and primary cells with carbon-nanotube transporters. Angew Chem Int Ed Engl 46:2023–2027 15. Yang R, Yang X, Zhang Z, Zhang Y, Wang S, Cai Z, Jia Y, Ma Y, Zheng C, Lu Y, Roden R, Chen Y (2006) Single-walled carbon nanotubes-mediated in vivo and in vitro delivery of siRNA into antigen-presenting cells.